model： JX-CKSM-12WK

brandShanghai Jingxin

specifications: Taiwan

Product IntroductionSingle cell suspension instrument (8-channel heating type) is a new generation of cell suspension preparation equipment that integrates metal bath digestion and mild tissue shearing. It is used in flow cytometry/single-cell sequencing/primary cell culture experiments and has the characteristics of small initial tissue volume, short time, high cell yield, and high cell activity. Designed using international DIN methods, low noise, easy to use, stable performance, with heating function, the digestion process of the sample is always in a constant temperature state.

Product Introduction:

The single-cell suspension instrument is a new generation of cell suspension preparation equipment that integrates metal bath digestion and mild tissue shearing. It is used in experiments such as flow cytometry, single-cell sequencing, and primary cell culture, and has the characteristics of small initial tissue volume, short time, high cell yield, and high cell activity. Designed using international DIN methods, low noise, easy to use, stable performance, with heating function, the digestion process of the sample is always in a constant temperature state.

working principle:

The sample is mixed with high-density zirconia shear magnetic beads and digestive enzymes, and then subjected to constant temperature digestion and tissue shear to obtain a high concentration of live cell suspension. After digestion termination, filtration and washing, and if necessary, red blood cell lysis steps can be performed to obtain millions to millions of live cells, and different tissues can achieve a cell yield of over 90%.

Sample range:

Application areas:Tumor research, cardiovascular research, stem cell research, immune research, neuroscience research

Product application:

Single Cell RNASeq sequencing

Multicolor Flow Cytometry

Mass Cytometry

Primary Cell Isolation and Culture

Cell therapy CAR-T

Advantages:

High utilization rate of the organization, efficient single-cell process, and high single-cell output

For clinical puncture samples, achieve a tissue utilization rate of 100% and a cell yield of over 100000

Complete the tissue to single-cell process within 15 minutes for the original cell culture sample, reduce cell stress time, and improve cell survival rate

For single-cell sequencing of tissues, rapid completion of single-cell processing to obtain high-yield and viability of over 85% single cells, taking mouse tissue as an example:

Operation steps:

Technical parameters: